PCR MCQ

Below PCR quiz are Multiple Choice Questions (MCQs) type Quiz. These PCR MCQ Questions helps you to refresh your PCR, you can see the correct option by clicking on it. .
  • 1. Starting with 15N15N (heavy) DNA, and after one generation in the 14N medium, Escherichia coli cells will contain

    • all 15N14N (hybrid) DNA.
    • half 15N15N DNA (heavy) and half 14N14N DNA (light).
    • half 14N14N (light) DNA and half 15N14N (hybrid) DNA.
    • all 14N14N (light) DNA.
  • 2. Which of the following statements are true regarding PCR

    • Automated PCR machines are called thermal cyclers
    • A thermostable DNA Polymerase is required
    • Billion copies of desired DNA can be synthesized from microgram quantities of DNA
    • All of these
  • 3. the basic requirements of PCR reaction include

    • DNA segment to be amplified
    • Two oligonucleotide primers
    • a heat stable DNA polymerase
    • all of these
  • 4. Which of the following statements about DNA replication is TRUE?

    • the leading strand is replicated continuously, while the lagging strand is replicated discontinuously
    • the leading strand is replicated discontinuously, while the lagging strand is replicated continuously
    • both the leading and lagging strands are replicated continuously
    • both the leading and lagging strands are replicated discontinuously
  • 5. You decide to use PCR to determine if you have Cystic Fibrosis, a genetic disorder. You then decide to also check if you are at risk for developing Alzheimers, another genetic disorder.What PCR "ingredient" would be different in these two tests?

    • Template DNA
    • Nucleotides
    • Taq DNA polymerase
    • Primers
  • 6. All of the following are thermostable polymerases except

    • Taq Polymerase
    • Vent Polymerase
    • DNA Polymerase III
    • pfu Polymerase